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Figure 5. Efficiency of <t>HDGF</t> suppression by <t>siRNA:</t> (a). Transfection of RLW cells with HDGF siRNA. HDGF was measured in cell lysates of HDGF- or control-siRNA-transfected RLW cells by immunoblot analysis (b) Quantification of immunoreactive bands by NIH ImageJ. Data are from 3 independent experiments presented as mean ± SEM. Bars with * are significantly different from each other (p ≤0.05).
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Figure 5. Efficiency of <t>HDGF</t> suppression by <t>siRNA:</t> (a). Transfection of RLW cells with HDGF siRNA. HDGF was measured in cell lysates of HDGF- or control-siRNA-transfected RLW cells by immunoblot analysis (b) Quantification of immunoreactive bands by NIH ImageJ. Data are from 3 independent experiments presented as mean ± SEM. Bars with * are significantly different from each other (p ≤0.05).
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Figure 5. Efficiency of HDGF suppression by siRNA: (a). Transfection of RLW cells with HDGF siRNA. HDGF was measured in cell lysates of HDGF- or control-siRNA-transfected RLW cells by immunoblot analysis (b) Quantification of immunoreactive bands by NIH ImageJ. Data are from 3 independent experiments presented as mean ± SEM. Bars with * are significantly different from each other (p ≤0.05).

Journal: International journal of molecular sciences

Article Title: Hepatocyte-Specific Triggering of Hepatic Stellate Cell Profibrotic Activation by Apoptotic Bodies: The Role of Hepatoma-Derived Growth Factor, HIV, and Ethanol.

doi: 10.3390/ijms24065346

Figure Lengend Snippet: Figure 5. Efficiency of HDGF suppression by siRNA: (a). Transfection of RLW cells with HDGF siRNA. HDGF was measured in cell lysates of HDGF- or control-siRNA-transfected RLW cells by immunoblot analysis (b) Quantification of immunoreactive bands by NIH ImageJ. Data are from 3 independent experiments presented as mean ± SEM. Bars with * are significantly different from each other (p ≤0.05).

Article Snippet: HDGF siRNA, sc-45878, was obtained from Santa Cruz Biotechnology, Dallas, TX, USA.

Techniques: Transfection, Control, Western Blot

Figure 9. Transfection of LX2 cells with siRNA. (a)The transfection efficiency of LX2 cells was measured by quantifying the siRNA-scrambled FITC (green)-positive cells. Cells were visualized by a 20× Keyence microscope. The picture used is representative data from one out of three independent experiments. (b) Transfection efficiency quantification.

Journal: International journal of molecular sciences

Article Title: Hepatocyte-Specific Triggering of Hepatic Stellate Cell Profibrotic Activation by Apoptotic Bodies: The Role of Hepatoma-Derived Growth Factor, HIV, and Ethanol.

doi: 10.3390/ijms24065346

Figure Lengend Snippet: Figure 9. Transfection of LX2 cells with siRNA. (a)The transfection efficiency of LX2 cells was measured by quantifying the siRNA-scrambled FITC (green)-positive cells. Cells were visualized by a 20× Keyence microscope. The picture used is representative data from one out of three independent experiments. (b) Transfection efficiency quantification.

Article Snippet: HDGF siRNA, sc-45878, was obtained from Santa Cruz Biotechnology, Dallas, TX, USA.

Techniques: Transfection, Microscopy